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1.0gm: Magnesium Sulfate: 0.1gm: Manganese Sulfate: 0.05gm: Final pH 6.5 +/- 0.3 at 25ºC. Procedure of MRS Broth Test. Inoculate MRS broth with an 18 to 24 hour culture of organism . Incubate the tube for 24 to 48 hours at 35°-37°C in ambient air. Examine the test tube for turbidity and gas production.

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Principle of MRS Broth Test. MRS Broth is an improved medium for lactobacilli, it supports good growth and is particularly useful for a number of fastidious strains which grow poorly on other general media. It was originally developed in 1960 by De Man, Rogosa & Sharpe, the medium can be used for confirmatory tests on organisms isolated on MRS ...

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